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10x polya polymerase buffer  (New England Biolabs)


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    Structured Review

    New England Biolabs 10x polya polymerase buffer
    10x Polya Polymerase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/10x+polya+polymerase+buffer/pmc08172540__41467_2021_23143_MOESM1_ESM-4-23-27
    Average 86 stars, based on 1 article reviews
    10x polya polymerase buffer - by Bioz Stars, 2026-10
    86/100 stars

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    other:

    Article Title: Discovery of widespread transcription initiation at microsatellites predictable by sequence-based deep neural network
    Article Snippet: Addition of polyA tail The polyA tail addition was carried out by using 8 ug of totalRNA in 14.5ul of water, 2.0ul of 10x PolyA polymerase buffer (NEB), 2.0ul of 10mM ATP (NEB), 1.0ul of RNaseOUT (Invitrogen) and 0.5ul of PolyA polymeras(5 U/ul).

    Article Title: Discovery of widespread transcription initiation at microsatellites predictable by sequence-based deep neural network
    Article Snippet: The polyA tail addition was carried out by using 8 ug of totalRNA in 14.5ul of water, 2.0ul of 10x PolyA polymerase buffer (NEB), 2.0ul of 10mM ATP (NEB), 1.0ul of RNaseOUT (Invitrogen) and 0.5ul of PolyA polymeras(5 U/ul).

    Incubation:

    Article Title: NERD-seq: A novel approach of Nanopore direct RNA sequencing that expands representation of non-coding RNAs
    Article Snippet: For NERD-seq, the standard protocol was further modified as follows: 1.5ug total RNA was separated into two fractions (short and long RNA) using the Invitrogen MirVana kit (AM1561) with a modified protocol as described before in our short RNA-seq approach for illumina ( – ). .. 90uL of the short RNA elution fraction was polyadenylated using 12uL 10x polyA polymerase buffer (NEB, B0276), 12uL 10mM ATP (NEB, B0756A), 6uL polyA polymerase (NEB, M0276) and incubated at 37C for 30min. .. The polyadenylated short fraction and the long fraction were combined and purified and concentrated using the RNeasy MinElute kit (Qiagen, 74204).



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    New England Biolabs 10x polya polymerase buffer
    10x Polya Polymerase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/10x+polya+polymerase+buffer/pmc08172540__41467_2021_23143_MOESM1_ESM-4-23-27
    Average 86 stars, based on 1 article reviews
    10x polya polymerase buffer - by Bioz Stars, 2026-10
    86/100 stars
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